H2N2V1, France, Analysis, bibRecord, 000262

Cold-sensitivity of polymerase complexes from human or avian influenza A viruses

Identifieur interne : 000262 ( France/Analysis ); précédent : 000261; suivant : 000263

Cold-sensitivity of polymerase complexes from human or avian influenza A viruses

Auteurs : Pascale Massin [France] ; Nadia Naffakh [France] ; Sylvie Van Der Werf [France]

Source :

International congress series [ 0531-5131 ] ; 2001.

RBID : ISTEX:B3B792E8CF5AD9D09BD63AFE024C516F24E6362F

English descriptors

Teeft :
- Avian, Avian influenza, Avian influenza viruses, Avian origin, Avian viruses, Cell extracts, Determinant, Human influenza viruses, Human virus, Human viruses, Influenza, Influenza virus, Influenza viruses, International congress series, Massin, Mdck, Mdck cells, Plaque assays, Polymerase, Polymerase complexes, Receptor specificity, Reconstituted rnps, Replication, Virol, Virus.

Abstract

Abstract: Background: Human influenza A viruses replicate in the upper respiratory tract at about 33 °C, whereas avian viruses replicate in the intestinal tract at a temperature close to 41 °C. Methods: The influence of low temperature on virus growth and RNA replication of avian and human viruses was analyzed in MDCK cells. The influence of temperature on the functional efficiency of homo- or heterospecific polymerase complexes derived from human or avian viruses, was examined by making use of a genetic system for the in vivo reconstitution of functional ribonucleoproteins. Results: Virus growth, RNA replication kinetics as well as the functional efficiency of the polymerase complex were found to be impaired at 33 °C as compared to 37 °C in the case of the avian A/FPV/Rostock/34 or A/Mallard/NY/6750/78 viruses, whereas they were comparable in the case of the human A/Puerto Rico/8/34 virus. The polymerase complex from the human virus A/Hong Kong/156/97 of avian origin exhibited an intermediate cold-sensitivity. The cold-sensitivity of the complexes of avian origin was determined mostly by residue 627 of PB2. Conclusions: Our results suggest that a reduced ability of the polymerase complex of avian viruses to ensure replication of the viral genome at 33 °C could contribute to their inability to grow efficiently in humans.

Url:

https://api.istex.fr/ark:/67375/6H6-W0TQWL0P-D/fulltext.pdf

DOI: 10.1016/S0531-5131(01)00399-5

Affiliations:

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ISTEX:B3B792E8CF5AD9D09BD63AFE024C516F24E6362F

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<front><div type="abstract" xml:lang="en">Abstract: Background: Human influenza A viruses replicate in the upper respiratory tract at about 33 °C, whereas avian viruses replicate in the intestinal tract at a temperature close to 41 °C. Methods: The influence of low temperature on virus growth and RNA replication of avian and human viruses was analyzed in MDCK cells. The influence of temperature on the functional efficiency of homo- or heterospecific polymerase complexes derived from human or avian viruses, was examined by making use of a genetic system for the in vivo reconstitution of functional ribonucleoproteins. Results: Virus growth, RNA replication kinetics as well as the functional efficiency of the polymerase complex were found to be impaired at 33 °C as compared to 37 °C in the case of the avian A/FPV/Rostock/34 or A/Mallard/NY/6750/78 viruses, whereas they were comparable in the case of the human A/Puerto Rico/8/34 virus. The polymerase complex from the human virus A/Hong Kong/156/97 of avian origin exhibited an intermediate cold-sensitivity. The cold-sensitivity of the complexes of avian origin was determined mostly by residue 627 of PB2. Conclusions: Our results suggest that a reduced ability of the polymerase complex of avian viruses to ensure replication of the viral genome at 33 °C could contribute to their inability to grow efficiently in humans.</div>
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Cold-sensitivity of polymerase complexes from human or avian influenza A viruses

Cold-sensitivity of polymerase complexes from human or avian influenza A viruses

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri